2jte Experiments and Validation

Solution NMR

Entry percentile scores
Source organism: Mus musculus
Refinement method: simulated annealing
Number of deposited models: 20 models
Chemical shifts: BMR15407  
Chemical shift assignment: 91%

Sample information

Author description:
Third SH3 domain of CD2AP
Source organism: Mus musculus
Expression system: Escherichia coli

Validation information

 
Structure ensemble information
Refinement method: simulated annealing
Number of calculated models: 300
Number of deposited models: 20 (structures with the lowest energy)
Representative model: 11 (medoid: most similar to other models)
 
Experimental NMR data
Chemical shifts: BMR15407  

Experimental information

Sample 1
Contents: 1 mM SH3-C, 1 mM [U-98% 15N] SH3-C, 1 mM [U-98% 13C; U-98% 15N] SH3-C, 90% H2O/10% D2O Recorded spectra: 2D 1H-15N HSQC, 3D 1H-15N NOESY, 3D 1H-15N NOESY, 3D 1H-15N TOCSY, 3D CBCA(CO)NH, 3D HBHA(CO)NH, 3D HCCH-TOCSY, 3D HNCACB, 3D HNCO

Sample 2
Contents: 1 mM SH3-C, 100% D2O Recorded spectra: 2D 1H-1H NOESY

Samples and spectra
NMR spectrometers used
Varian Uniform NMR System 600 MHz, Varian INOVA 800 MHz