› 2jsc

Solution NMR

NMR structure of the cadmium metal-sensor CMTR from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb2jsc/pdb
PDB entry 2jsc coloured by chain, ensemble of 30 models, front view.
PDB entry 2jsc coloured by chain, ensemble of 30 models, side view.
PDB entry 2jsc coloured by chain, ensemble of 30 models, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
NMR structural analysis of cadmium sensing by winged helix repressor CmtR.
Banci L, Bertini I, Cantini F, Ciofi-Baffoni S, Cavet JS, Dennison C, Graham AI, Harvie DR, Robinson NJ
J Biol Chem 282 30181-8 (2007)
PMID: 17599915

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator CmtR Chains: A, B
Molecule details ›
Chains: A, B
Length: 118 amino acids
Theoretical weight: 12.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMI9 (Residues: 1-118; Coverage: 100%)
Gene names: MTCY39.25, Rv1994c, cmtR
Sequence domains: Bacterial regulatory protein, arsR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand CD 2 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE DYNAMICS COUPLED WITH SIMULATED ANNEALING FOLLOWED BY RESTRAINED ENERGY MINIMIZATION
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
6 more

5 mentions without citation

Solution NMR Studies of Mycobacterium tuberculosis Proteins for Antibiotic Target Discovery.
Kim et al. (2017)
4 more