PDBe 2jke

X-ray diffraction
1.7Å resolution

Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin


Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Glucan 1,4-alpha-glucosidase SusB Chains: A, B
Molecule details ›
Chains: A, B
Length: 727 amino acids
Theoretical weight: 83.31 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
  • Canonical: G8JZS4 (Residues: 22-738; Coverage: 100%)
Gene names: BT_3703, susB
Sequence domains:
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 75.722Å b: 112.244Å c: 102.494Å
α: 90° β: 100.86° γ: 90°
R R work R free
0.157 0.155 0.187
Expression system: Escherichia coli BL21