› 2jkd

X-ray diffraction
2.5Å resolution

Structure of the yeast Pml1 splicing factor and its integration into the RES complex

Released:
DOI: 10.2210/pdb2jkd/pdb
PDB entry 2jkd coloured by chain, front view.
PDB entry 2jkd coloured by chain, side view.
PDB entry 2jkd coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the yeast Pml1 splicing factor and its integration into the RES complex.
Brooks MA, Dziembowski A, Quevillon-Cheruel S, Henriot V, Faux C, van Tilbeurgh H, Séraphin B
Nucleic Acids Res 37 129-43 (2009)
PMID: 19033360

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA leakage protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 187 amino acids
Theoretical weight: 21.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07930 (Residues: 24-204; Coverage: 89%)
Gene names: L1591, PML1, YLR016C
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 85.72Å b: 85.72Å c: 97.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.262
Expression system: Escherichia coli

Quick links

Citations

30 citation in other articles

Molecular architecture of the Saccharomyces cerevisiae activated spliceosome.
Rauhut et al. (2016)
29 more

1 mention without citation

Structure of the yeast Pml1 splicing factor and its integration into the RES complex.
Brooks et al. (2009)