PDBe 2jkb

X-ray diffraction
1.54Å resolution

Crystal structure of Streptococcus pneumoniae NanB in complex with 2, 7-anhydro-Neu5Ac


Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Sialidase B Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 76.74 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21
  • Canonical: Q54727 (Residues: 22-29, 30-697; Coverage: 100%)
Gene names: SP_1687, nanB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 76.719Å b: 82.746Å c: 118.079Å
α: 90° β: 90° γ: 90°
R R work R free
0.163 0.162 0.188
Expression system: Escherichia coli BL21