› 2jgn

X-ray diffraction
1.91Å resolution

DDX3 helicase domain

Released:
DOI: 10.2210/pdb2jgn/pdb
PDB entry 2jgn coloured by chain, front view.
PDB entry 2jgn coloured by chain, side view.
PDB entry 2jgn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Expression, purification, crystallization and preliminary X-ray diffraction analysis of the DDX3 RNA helicase domain.
Rodamilans B, Montoya G
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 283-6 (2007)
PMID: 17401195

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DDX3X Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 185 amino acids
Theoretical weight: 21.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00571 (Residues: 409-580; Coverage: 26%)
Gene names: DBX, DDX3, DDX3X
Sequence domains: Helicase conserved C-terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 44.438Å b: 61.032Å c: 89.111Å
α: 90° β: 101.67° γ: 90°
R-values:
R R work R free
0.201 0.198 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Ded1/DDX3 subfamily of DEAD-box RNA helicases.
Sharma et al. (2014)
1 more

8 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
7 more