PDBe 2jec

X-ray diffraction
2Å resolution

crystal structure of recombinant DiocleA grandiflora lectin mutant E123A-H131N-K132Q complexed witH 5-bromo-4-chloro-3-indolyl-a-D- mannose

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lectin alpha chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 239 amino acids
Theoretical weight: 25.75 KDa
Source organism: Dioclea grandiflora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08902 (Residues: 1-237; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 72.194Å b: 84.552Å c: 176.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.23
Expression system: Escherichia coli BL21(DE3)