› 2jbf

X-ray diffraction
1.7Å resolution

Structure of PBP-A, L158E mutant. Acyl-enzyme complex with penicillin- G.

Released:
DOI: 10.2210/pdb2jbf/pdb
PDB entry 2jbf coloured by chain, front view.
PDB entry 2jbf coloured by chain, side view.
PDB entry 2jbf coloured by chain, top view.
Source organism: Synechococcus elongatus
Primary publication:
Structure of PBP-A from Thermosynechococcus elongatus, a penicillin-binding protein closely related to class A beta-lactamases.
Urbach C, Evrard C, Pudzaitis V, Fastrez J, Soumillion P, Declercq JP
J Mol Biol 386 109-20 (2009)
PMID: 19100272

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase class A catalytic domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 298 amino acids
Theoretical weight: 32.82 KDa
Source organism: Synechococcus elongatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DH45 (Residues: 93-368; Coverage: 75%)
Gene name: tll2115
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand PNM 4 x PNM
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 86.579Å b: 91.525Å c: 145.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.218
Expression system: Escherichia coli

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Citations

1 review citation

Epidemiological expansion, structural studies, and clinical challenges of new β-lactamases from gram-negative bacteria.
Bush et al. (2011)
9 other articles