PDBe 2jam

X-ray diffraction
1.7Å resolution

Crystal structure of human calmodulin-dependent protein kinase I G

Released:
Source organisms:
Entry authors: Debreczeni JE, Bullock A, Keates T, Niesen FH, Salah E, Shrestha L, Smee C, Sobott F, Pike ACW, Bunkoczi G, von Delft F, Turnbull A, Weigelt J, Arrowsmith CH, Edwards A, Sundstrom M, Knapp S

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
POLYPEPTIDE Chain: D
Molecule details ›
Chain: D
Length: 6 amino acids
Theoretical weight: 609 Da
Source organism: unidentified
POLYPEPTIDE Chain: E
Molecule details ›
Chain: E
Length: 4 amino acids
Theoretical weight: 481 Da
Source organism: unidentified
Calcium/calmodulin-dependent protein kinase type 1G Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 34.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96NX5 (Residues: 176-316; Coverage: 39%)
Gene names: CAMK1G, CLICK3, VWS1
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 51.666Å b: 53.736Å c: 64.177Å
α: 65.45° β: 71.91° γ: 73.84°
R-values:
R R work R free
0.157 0.155 0.231
Expression system: Escherichia coli BL21(DE3)