› 2j8g

X-ray diffraction
1.69Å resolution

Crystal structure of the modular Cpl-1 endolysin complexed with a peptidoglycan analogue (E94Q mutant in complex with a tetrasaccharide- pentapeptide)

Released:
DOI: 10.2210/pdb2j8g/pdb
PDB entry 2j8g coloured by chain, front view.
PDB entry 2j8g coloured by chain, side view.
PDB entry 2j8g coloured by chain, top view.
Source organism: Streptococcus phage Cp1
Primary publication:
Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1.
Pérez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Páez JA, García P, Martínez-Ripoll M, García JL, Mobashery S, Menéndez M, Hermoso JA
J Biol Chem 282 24990-9 (2007)
PMID: 17581815

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysozyme Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 39.22 KDa
Source organism: Streptococcus phage Cp1
Expression system: Escherichia coli DH1
UniProt:
  • Canonical: P15057 (Residues: 1-339; Coverage: 100%)
Gene names: 22, CPL1
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, AMU
Carbohydrate polymer
4 bound ligands:
Ligand ALA 1 x ALA
Ligand DGL 1 x DGL
Ligand LYS 1 x LYS
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 79.52Å b: 97.39Å c: 127.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.22
Expression system: Escherichia coli DH1

Quick links

Citations

9 review citations

Taking aim on bacterial pathogens: from phage therapy to enzybiotics.
Hermoso et al. (2007)
8 more

5 mentions without citation

Choline Binding Proteins from Streptococcus pneumoniae: A Dual Role as Enzybiotics and Targets for the Design of New Antimicrobials.
Maestro et al. (2016)
4 more