PDBe 2j66

X-ray diffraction
1.65Å resolution

Structural characterisation of BtrK decarboxylase from butirosin biosynthesis

Released:
Source organism: Bacillus circulans
Entry authors: Popovic B, Li Y, Chirgadze DY, Blundell TL, Spencer JB

Function and Biology Details

Reaction catalysed:
L-glutamyl-[BtrI acyl-carrier protein] = 4-amino butanoyl-[BtrI acyl-carrier protein] + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-glutamyl-[BtrI acyl-carrier protein] decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 428 amino acids
Theoretical weight: 47.87 KDa
Source organism: Bacillus circulans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2L4H3 (Residues: 1-428; Coverage: 100%)
Gene name: btrK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P21212
Unit cell:
a: 71.604Å b: 128.814Å c: 45.323Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.224
Expression system: Escherichia coli