PDBe 2j59

X-ray diffraction
2.1Å resolution

Crystal structure of the ARF1:ARHGAP21-ArfBD complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 166 amino acids
Theoretical weight: 18.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84078 (Residues: 18-181; Coverage: 91%)
Gene name: Arf1
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Rho GTPase-activating protein 21 Chains: M, N, O, P, Q, R
Molecule details ›
Chains: M, N, O, P, Q, R
Length: 168 amino acids
Theoretical weight: 19.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5T5U3 (Residues: 929-1096; Coverage: 9%)
Gene names: ARHGAP10, ARHGAP21, KIAA1424
Sequence domains: Pleckstrin homology domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 74.636Å b: 132.136Å c: 146.283Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.209 0.205 0.243
Expression system: Escherichia coli