› 2j46

X-ray diffraction
1.14Å resolution

Water structure of T. Aquaticus Ffh NG Domain At 1.1A Resolution

Released:
DOI: 10.2210/pdb2j46/pdb
PDB entry 2j46 coloured by chain, front view.
PDB entry 2j46 coloured by chain, side view.
PDB entry 2j46 coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Analysis of protein hydration in ultrahigh-resolution structures of the SRP GTPase Ffh.
Ramirez UD, Freymann DM
Acta Crystallogr D Biol Crystallogr 62 1520-34 (2006)
PMID: 17139088

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 32.57 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: O07347 (Residues: 1-297; Coverage: 69%)
Gene name: ffh
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MRD 2 x MRD
Ligand CL 4 x CL
Ligand ACT 2 x ACT
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 109.473Å b: 54.496Å c: 98.799Å
α: 90° β: 97.72° γ: 90°
R-values:
R R work R free
0.15 0.149 0.172
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

X-ray-radiation-induced cooperative atomic movements in protein.
Petrova et al. (2009)
2 more

2 mentions without citation

Predicting binding sites from unbound versus bound protein structures.
Clark et al. (2020)
1 more