PDBe 2j25

X-ray diffraction
2.9Å resolution

Partially deglycosylated glucoceramidase

Released:

Function and Biology Details

Reaction catalysed:
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosylceramidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 55.64 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GC, GLUC
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 108.626Å b: 280.821Å c: 91.064Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.273
Expression system: Cricetulus griseus