› 2iyn

X-ray diffraction
2.08Å resolution

The co-factor-induced pre-active conformation in PhoB

Released:
DOI: 10.2210/pdb2iyn/pdb
PDB entry 2iyn coloured by chain, front view.
PDB entry 2iyn coloured by chain, side view.
PDB entry 2iyn coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The cofactor-induced pre-active conformation in PhoB.
Solà M, Drew DL, Blanco AG, Gomis-Rüth FX, Coll M
Acta Crystallogr D Biol Crystallogr 62 1046-57 (2006)
PMID: 16929106

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate regulon transcriptional regulatory protein PhoB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 127 amino acids
Theoretical weight: 14.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0AFJ5 (Residues: 1-127; Coverage: 56%)
Gene names: JW0389, b0399, phoB
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: C2221
Unit cell:
a: 50.742Å b: 105.829Å c: 135.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.305
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Global regulation by the seven-component Pi signaling system.
Hsieh et al. (2010)
1 more

3 mentions without citation

A link between dimerization and autophosphorylation of the response regulator PhoB.
Creager-Allen et al. (2013)
2 more