PDBe 2ixs

X-ray diffraction
2Å resolution

Structure of SdaI restriction endonuclease

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
R.SdaI Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.53 KDa
Source organism: Streptomyces diastaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: C4P954 (Residues: 1-323; Coverage: 100%)
Gene name: sdaIR
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: C2221
Unit cell:
a: 66.901Å b: 237.991Å c: 111.404Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.178 0.212
Expression system: Escherichia coli