› 2iwi

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF THE HUMAN PIM2 IN COMPLEX WITH A RUTHENIUM ORGANOMETALLIC LIGAND RU1

Released:
DOI: 10.2210/pdb2iwi/pdb
PDB entry 2iwi coloured by chain, front view.
PDB entry 2iwi coloured by chain, side view.
PDB entry 2iwi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the PIM2 kinase in complex with an organoruthenium inhibitor.
Bullock AN, Russo S, Amos A, Pagano N, Bregman H, Debreczeni JE, Lee WH, von Delft F, Meggers E, Knapp S
PLoS One 4 e7112 (2009)
PMID: 19841674

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase pim-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 34.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9P1W9 (Residues: 1-311; Coverage: 100%)
Gene name: PIM2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand HB1 2 x HB1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: R3
Unit cell:
a: 153.07Å b: 153.07Å c: 78.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.242 0.289
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

PIM serine/threonine kinases in the pathogenesis and therapy of hematologic malignancies and solid cancers.
Brault et al. (2010)
10 more

6 mentions without citation

Crystal structure of the PIM2 kinase in complex with an organoruthenium inhibitor.
Bullock et al. (2009)
5 more