PDBe 2iui

X-ray diffraction
2.4Å resolution

Crystal structure of the PI3-kinase p85 N-terminal SH2 domain in complex with PDGFR phosphotyrosyl peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PDGFR PHOSPHOTYROSYL PEPTIDE Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.39 KDa
Source organism: Unidentified
Expression system: Escherichia coli
Phosphatidylinositol 3-kinase regulatory subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 120 amino acids
Theoretical weight: 13.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27986 (Residues: 321-440; Coverage: 17%)
  • Best match: P27986-5 (Residues: 1-77)
Gene names: GRB1, PIK3R1
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 40.76Å b: 61.94Å c: 108.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.248
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)