PDBe 2iuh

X-ray diffraction
2Å resolution

Crystal structure of the PI3-kinase p85 N-terminal SH2 domain in complex with c-Kit phosphotyrosyl peptide


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biological process:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mast/stem cell growth factor receptor Kit Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P10721 (Residues: 718-728; Coverage: 1%)
Gene names: KIT, SCFR
Phosphatidylinositol 3-kinase regulatory subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P27986 (Residues: 321-440; Coverage: 17%)
  • Best match: P27986-5 (Residues: 1-77)
Gene names: GRB1, PIK3R1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 56.95Å b: 56.95Å c: 68.17Å
α: 90° β: 90° γ: 90°
R R work R free
0.154 0.154 0.245
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)