PDBe 2ihy

X-ray diffraction
1.9Å resolution

Structure of the Staphylococcus aureus putative ATPase subunit of an ATP-binding cassette (ABC) transporter

Released:
Source organism: Staphylococcus aureus
Entry authors: McGrath TE, Yu CS, Romanov V, Lam R, Dharamsi A, Virag C, Mansoury K, Thambipillai D, Richards D, Guthrie J, Edwards AM, Pai EF, Chirgadze NY

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + iron chelate(Out) = ADP + phosphate + iron chelate(In)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABC transporter, ATP-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 31.55 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2WZ98 (Residues: 1-260; Coverage: 100%)
Gene name: SACOL2144
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 51.972Å b: 74.832Å c: 68.818Å
α: 90° β: 102.21° γ: 90°
R-values:
R R work R free
0.193 0.191 0.231
Expression system: Escherichia coli BL21(DE3)