2ifs

Solution NMR

Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-126691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin nucleation-promoting factor WASL Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O08816 (Residues: 1-22, 23-147; Coverage: 29%)
Gene name: Wasl
Sequence domains: WH1 domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT. FINAL STRUCTURES WERE OBTAINED BY MOLECULAR DYNAMICS IN EXPLICIT SOLVENT
Expression system: Escherichia coli BL21(DE3)