PDBe 2ian

X-ray diffraction
2.8Å resolution

Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
T-cell receptor beta-1 chain C region Chains: E, J, O, T
Molecule details ›
Chains: E, J, O, T
Length: 240 amino acids
Theoretical weight: 27.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01850 (Residues: 1-130; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T-cell receptor alpha chain C region Chains: D, I, N, S
Molecule details ›
Chains: D, I, N, S
Length: 202 amino acids
Theoretical weight: 22.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01848 (Residues: 1-95; Coverage: 67%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
Triosephosphate isomerase Chains: C, H, M, R
Molecule details ›
Chains: C, H, M, R
Length: 15 amino acids
Theoretical weight: 1.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P60174 (Residues: 60-74; Coverage: 5%)
Gene names: TPI, TPI1
HLA class II histocompatibility antigen, DR alpha chain Chains: A, F, K, P
Molecule details ›
Chains: A, F, K, P
Length: 182 amino acids
Theoretical weight: 21.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1-1 beta chain Chains: B, G, L, Q
Molecule details ›
Chains: B, G, L, Q
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04229 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 93.474Å b: 242.866Å c: 105.222Å
α: 90° β: 111.32° γ: 90°
R-values:
R R work R free
0.225 0.221 0.296
Expression system: Escherichia coli BL21