› 2i0e

X-ray diffraction
2.6Å resolution

Structure of catalytic domain of human protein kinase C beta II complexed with a bisindolylmaleimide inhibitor

Released:
DOI: 10.2210/pdb2i0e/pdb
PDB entry 2i0e coloured by chain, front view.
PDB entry 2i0e coloured by chain, side view.
PDB entry 2i0e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the catalytic domain of human protein kinase C beta II complexed with a bisindolylmaleimide inhibitor.
Grodsky N, Li Y, Bouzida D, Love R, Jensen J, Nodes B, Nonomiya J, Grant S
Biochemistry 45 13970-81 (2006)
PMID: 17115692

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-138768 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C beta type Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 40.8 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P05771 (Residues: 321-668; Coverage: 52%)
Gene names: PKCB, PRKCB, PRKCB1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PDS 2 x PDS
2 modified residues:
Ligand TPO 4 x TPO
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21212
Unit cell:
a: 93.098Å b: 131.417Å c: 83.801Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.29
Expression system: Spodoptera frugiperda

Quick links

Citations

21 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
20 more

38 mentions without citation

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Rodríguez et al. (2022)
37 more