› 2hxy

X-ray diffraction
3.3Å resolution

Crystal structure of human apo-eIF4AIII

Released:
DOI: 10.2210/pdb2hxy/pdb
PDB entry 2hxy coloured by chain, front view.
PDB entry 2hxy coloured by chain, side view.
PDB entry 2hxy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.
Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Séraphin B, Le Hir H, Andersen GR
Science 313 1968-72 (2006)
PMID: 16931718

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153768 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic initiation factor 4A-III Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 391 amino acids
Theoretical weight: 44.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38919 (Residues: 23-411; Coverage: 95%)
Gene names: DDX48, EIF4A3, KIAA0111
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 150.22Å b: 238.05Å c: 79.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.276 0.275 0.308
Expression system: Escherichia coli

Quick links

Citations

83 review citations

The mechanism of eukaryotic translation initiation and principles of its regulation.
Jackson et al. (2010)
82 more

9 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
8 more