› 2hug

Solution NMR

3D Solution Structure of the Chromo-2 Domain of cpSRP43 complexed with cpSRP54 peptide

Released:
DOI: 10.2210/pdb2hug/pdb
PDB entry 2hug coloured by chain, ensemble of 20 models, front view.
PDB entry 2hug coloured by chain, ensemble of 20 models, side view.
PDB entry 2hug coloured by chain, ensemble of 20 models, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Assembly of chloroplast signal recognition particle involves structural rearrangement in cpSRP43.
Kathir KM, Rajalingam D, Sivaraja V, Kight A, Goforth RL, Yu C, Henry R, Kumar TK
J Mol Biol 381 49-60 (2008)
PMID: 18586266

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127624 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Signal recognition particle 43 kDa protein, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 57 amino acids
Theoretical weight: 6.55 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O22265 (Residues: 265-319; Coverage: 15%)
Gene names: At2g47450, CAO, CPSRP43, T30B22.25
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Signal recognition particle subunit SRP54, chloroplastic Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.52 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P37107 (Residues: 530-543; Coverage: 3%)
Gene names: At5g03940, CPSRP54, F8F6_150, FFC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 69%
Refinement method: distance geometry, simulated annealing, molecular dynamics, torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
4 more

1 mention without citation

Assembly of chloroplast signal recognition particle involves structural rearrangement in cpSRP43.
Kathir et al. (2008)