PDBe 2htf

Solution NMR

The solution structure of the BRCT domain from human polymerase reveals homology with the TdT BRCT domain

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed DNA/RNA polymerase mu Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NP87 (Residues: 21-124; Coverage: 21%)
Gene names: POLM, polmu
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: Torsion angle dynamics followed by molecular dynamics refinement in explicit water solvent
Chemical shifts: BMR7259  
Expression system: Escherichia coli