› 2hte

X-ray diffraction
2Å resolution

The crystal structure of spermidine synthase from p. falciparum in complex with 5'-methylthioadenosine

Released:
DOI: 10.2210/pdb2hte/pdb
PDB entry 2hte coloured by chain, front view.
PDB entry 2hte coloured by chain, side view.
PDB entry 2hte coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms.
Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R
Mol Biochem Parasitol 151 100-10 (2007)
PMID: 17125854

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PABS domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 283 amino acids
Theoretical weight: 32.22 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8II73 (Residues: 40-321; Coverage: 88%)
Gene name: PF3D7_1129000
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand UNX 13 x UNX
Ligand MTA 3 x MTA
Ligand 1PG 2 x 1PG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 197.061Å b: 134.6Å c: 48.336Å
α: 90° β: 94.66° γ: 90°
R-values:
R R work R free
0.192 0.186 0.231
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Structure-based insights into the catalytic power and conformational dexterity of peroxiredoxins.
Hall et al. (2011)
32 more

7 mentions without citation

Polyamines in protozoan pathogens.
Phillips MA. (2018)
6 more