PDBe 2hsa

X-ray diffraction
1.5Å resolution

Crystal structure of 12-oxophytodienoate reductase 3 (OPR3) from tomato

Released:

Function and Biology Details

Reaction catalysed:
8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP(+) = (15Z)-12-oxophyto-10,15-dienoate + NADPH. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
12-oxophytodienoate reductase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 402 amino acids
Theoretical weight: 44.4 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FEW9 (Residues: 1-396; Coverage: 100%)
Gene name: OPR3
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 57.904Å b: 89.677Å c: 81.378Å
α: 90° β: 109.46° γ: 90°
R-values:
R R work R free
0.225 0.225 0.255
Expression system: Escherichia coli