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Crystal Structure of E. coli PepN (Aminopeptidase N)in complex with Bestatin

Source organism: Escherichia coli K-12
Biochemical function: metal ion binding
Biological process: proteolysis
Cellular component: membrane

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EC 3.4.11.2: Membrane alanyl aminopeptidase

Reaction catalysed:
Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.
Systematic name:
-
Alternative Name(s):
  • Alanine aminopeptidase
  • Alanine-specific aminopeptidase
  • Alanyl aminopeptidase
  • Amino-oligopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • CD13
  • Cysteinylglycinase
  • Cysteinylglycine dipeptidase
  • L-alanine aminopeptidase
  • Membrane alanine aminopeptidase
  • Membrane aminopeptidase I
  • Microsomal aminopeptidase
  • Particle-bound aminopeptidase
  • Peptidase E
  • Pseudo leucine aminopeptidase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF17432
Domain description: Domain of unknown function (DUF3458_C) ARM repeats
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of Pfam domain PF17432 (Domain of unknown function (DUF3458_C) ARM repeats) in Aminopeptidase N. Showing 1 copy in chain A.

PF11940
Domain description: Domain of unknown function (DUF3458) Ig-like fold
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of Pfam domain PF11940 (Domain of unknown function (DUF3458) Ig-like fold) in Aminopeptidase N. Showing 1 copy in chain A.

PF01433
Domain description: Peptidase family M1 domain
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of Pfam domain PF01433 (Peptidase family M1 domain) in Aminopeptidase N. Showing 1 copy in chain A.

PF17900
Domain description: Peptidase M1 N-terminal domain
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of Pfam domain PF17900 (Peptidase M1 N-terminal domain) in Aminopeptidase N. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR001930
Domain description: Peptidase M1, alanine aminopeptidase/leukotriene A4 hydrolase
Occurring in:
  1. Aminopeptidase N
IPR012779
Domain description: Peptidase M1, alanyl aminopeptidase
Occurring in:
  1. Aminopeptidase N
IPR042097
Domain description: Aminopeptidase N-like , N-terminal domain superfamliy
Occurring in:
  1. Aminopeptidase N
IPR024601
Domain description: Peptidase M1, alanyl aminopeptidase, C-terminal
Occurring in:
  1. Aminopeptidase N
IPR037144
Domain description: Peptidase M1, alanyl aminopeptidase, C-terminal domain superfamily
Occurring in:
  1. Aminopeptidase N
IPR038438
Domain description: Alanyl aminopeptidase, Ig-like domain superfamily
Occurring in:
  1. Aminopeptidase N
IPR035414
Domain description: Peptidase M1, alanyl aminopeptidase, Ig-like fold
Occurring in:
  1. Aminopeptidase N
IPR027268
Domain description: Peptidase M4/M1, CTD superfamily
Occurring in:
  1. Aminopeptidase N
IPR014782
Domain description: Peptidase M1, membrane alanine aminopeptidase
Occurring in:
  1. Aminopeptidase N
IPR045357
Domain description: Aminopeptidase N-like , N-terminal domain
Occurring in:
  1. Aminopeptidase N

Structure domains

CATH CATH domains
1.25.50.10
Class: Mainly Alpha
Architecture: Alpha Horseshoe
Topology: Zincin-like fold
Homology: Peptidase M1, alanyl aminopeptidase, C-terminal domain
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of CATH domain 1.25.50.10 (Zincin-like fold) in Aminopeptidase N. Showing 1 copy in chain A.
2.60.40.1840
Class: Mainly Beta
Architecture: Sandwich
Topology: Immunoglobulin-like
Homology: Immunoglobulin-like
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of CATH domain 2.60.40.1840 (Immunoglobulin-like) in Aminopeptidase N. Showing 1 copy in chain A.
1.10.390.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Neutral Protease; domain 2
Homology: Neutral Protease Domain 2
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of CATH domain 1.10.390.10 (Neutral Protease; domain 2) in Aminopeptidase N. Showing 1 copy in chain A.
3.30.2010.30
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Zincin-like
Homology: Zincin-like
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of CATH domain 3.30.2010.30 (Zincin-like) in Aminopeptidase N. Showing 1 copy in chain A.
2.60.40.1730
Class: Mainly Beta
Architecture: Sandwich
Topology: Immunoglobulin-like
Homology: tricorn interacting facor f3 domain
Occurring in:
  1. Aminopeptidase N
The deposited structure of PDB entry 2hpt contains 1 copy of CATH domain 2.60.40.1730 (Immunoglobulin-like) in Aminopeptidase N. Showing 1 copy in chain A.

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