› 2hoy

X-ray diffraction
2.2Å resolution

Inter-subunit signaling in GSAM

Released:
DOI: 10.2210/pdb2hoy/pdb
PDB entry 2hoy coloured by chain, front view.
PDB entry 2hoy coloured by chain, side view.
PDB entry 2hoy coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 6301
Primary publication:
Intersubunit signaling in glutamate-1-semialdehyde-aminomutase.
Stetefeld J, Jenny M, Burkhard P
Proc Natl Acad Sci U S A 103 13688-93 (2006)
PMID: 16954186

Function and Biology Details

Reaction catalysed: 
(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate-1-semialdehyde 2,1-aminomutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 432 amino acids
Theoretical weight: 46.06 KDa
Source organism: Synechococcus elongatus PCC 6301
Expression system: Escherichia coli
UniProt:
  • Canonical: P24630 (Residues: 2-433; Coverage: 100%)
Gene names: gsa, hemL, syc0881_c
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 65.99Å b: 107.084Å c: 122.053Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.248
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The biochemistry of heme biosynthesis.
Heinemann et al. (2008)
3 more

1 mention without citation

Intersubunit signaling in glutamate-1-semialdehyde-aminomutase.
Stetefeld et al. (2006)