2hj6 › Citations

Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides.

Roszak AW, Gardiner AT, Isaacs NW, Cogdell RJ
Biochemistry 46 2909-16 (2007)
Related entries: 2hg3, 2hg9, 2hh1, 2hhk, 2hit

Cited: 11 times
EuropePMC logo PMID: 17315985

Abstract

This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.

Articles - 2hj6 mentioned but not cited (1)

  1. Prediction of Ordered Water Molecules in Protein Binding Sites from Molecular Dynamics Simulations: The Impact of Ligand Binding on Hydration Networks. Rudling A, Orro A, Carlsson J. J Chem Inf Model 58 350-361 (2018)


Reviews citing this publication (5)

  1. Overcoming barriers to membrane protein structure determination. Bill RM, Henderson PJ, Iwata S, Kunji ER, Michel H, Neutze R, Newstead S, Poolman B, Tate CG, Vogel H. Nat Biotechnol 29 335-340 (2011)
  2. Gating and regulation of the cytochrome c oxidase proton pump. Ferguson-Miller S, Hiser C, Liu J. Biochim Biophys Acta 1817 489-494 (2012)
  3. Conserved lipid-binding sites in membrane proteins: a focus on cytochrome c oxidase. Qin L, Sharpe MA, Garavito RM, Ferguson-Miller S. Curr Opin Struct Biol 17 444-450 (2007)
  4. Solid-state NMR of nanomachines involved in photosynthetic energy conversion. Alia A, Buda F, de Groot HJ, Matysik J. Annu Rev Biophys 42 675-699 (2013)
  5. Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins. Hanashima S, Nakane T, Mizohata E. Membranes (Basel) 11 823 (2021)

Articles citing this publication (5)

  1. Extension of the GLYCAM06 Biomolecular Force Field to Lipids, Lipid Bilayers and Glycolipids. Tessier MB, Demarco ML, Yongye AB, Woods RJ. Mol Simul 34 349-363 (2008)
  2. Cardiolipin-based respiratory complex activation in bacteria. Arias-Cartin R, Grimaldi S, Pommier J, Lanciano P, Schaefer C, Arnoux P, Giordano G, Guigliarelli B, Magalon A. Proc Natl Acad Sci U S A 108 7781-7786 (2011)
  3. Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent. Nakane T, Hanashima S, Suzuki M, Saiki H, Hayashi T, Kakinouchi K, Sugiyama S, Kawatake S, Matsuoka S, Matsumori N, Nango E, Kobayashi J, Shimamura T, Kimura K, Mori C, Kunishima N, Sugahara M, Takakyu Y, Inoue S, Masuda T, Hosaka T, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Inoue T, Nureki O, Iwata S, Murata M, Mizohata E. Proc Natl Acad Sci U S A 113 13039-13044 (2016)
  4. New insights into the structure of the reaction centre from Blastochloris viridis: evolution in the laboratory. Roszak AW, Moulisová V, Reksodipuro AD, Gardiner AT, Fujii R, Hashimoto H, Isaacs NW, Cogdell RJ. Biochem J 442 27-37 (2012)
  5. Lipid binding to the carotenoid binding site in photosynthetic reaction centers. Deshmukh SS, Tang K, Kálmán L. J Am Chem Soc 133 16309-16316 (2011)


Quick links