› 2hik

X-ray diffraction
3.3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-157629 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA polymerase sliding clamp 1 Chains: A, L, X
Molecule details ›
Chains: A, L, X
Length: 257 amino acids
Theoretical weight: 29.06 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P57766 (Residues: 1-249; Coverage: 100%)
Gene names: C41_008, SSO0397, pcn1, pcnA-like
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase sliding clamp 2 Chains: B, M, Y
Molecule details ›
Chains: B, M, Y
Length: 245 amino acids
Theoretical weight: 27.65 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Z84 (Residues: 1-245; Coverage: 100%)
Gene names: SSO1047, pcn2, pcnA-2
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase sliding clamp 3 Chains: C, N, Z
Molecule details ›
Chains: C, N, Z
Length: 252 amino acids
Theoretical weight: 28.75 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P57765 (Residues: 1-244; Coverage: 100%)
Gene names: C41_016, SSO0405, pcn3, pcnA-1
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 51 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P21212
Unit cell:
a: 142.275Å b: 223.093Å c: 79.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.236 0.299
Expression system: Escherichia coli

Quick links

Citations

28 review citations

PCNA, the maestro of the replication fork.
Moldovan et al. (2007)
27 more

2 mentions without citation

Archaeal genome guardians give insights into eukaryotic DNA replication and damage response proteins.
Shin et al. (2014)
1 more