› 2hgt

X-ray diffraction
2.2Å resolution

STRUCTURE OF THE HIRUGEN AND HIRULOG 1 COMPLEXES OF ALPHA-THROMBIN

Released:
DOI: 10.2210/pdb2hgt/pdb
PDB entry 2hgt coloured by chain, front view.
PDB entry 2hgt coloured by chain, side view.
PDB entry 2hgt coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the hirugen and hirulog 1 complexes of alpha-thrombin.
Skrzypczak-Jankun E, Carperos VE, Ravichandran KG, Tulinsky A, Westbrook M, Maraganore JM
J Mol Biol 221 1379-93 (1991)
PMID: 1942057

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133088 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Thrombin light chain Chain: L
Molecule details ›
Chain: L
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
Sequence domains: Thrombin light chain
Thrombin heavy chain Chain: H
Molecule details ›
Chain: H
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Hirulog Chain: I
Molecule details ›
Chain: I
Length: 3 amino acids
Theoretical weight: 419 Da
Hirudin-2 Chain: J
Molecule details ›
Chain: J
Length: 17 amino acids
Theoretical weight: 1.78 KDa
Source organism: Hirudo medicinalis
Expression system: Not provided
UniProt:
  • Canonical: P28504 (Residues: 48-64; Coverage: 26%)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand DPN 1 x DPN

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 70.65Å b: 72.4Å c: 72.82Å
α: 90° β: 100.76° γ: 90°
R-values:
R R work R free
0.163 not available not available
Expression system: Not provided

Quick links

Citations

31 review citations

Parenteral anticoagulants: Antithrombotic Therapy and Prevention of Thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines.
Garcia et al. (2012)
30 more

1 mention without citation

Crystal structure of thrombin in complex with S-variegin: insights of a novel mechanism of inhibition and design of tunable thrombin inhibitors.
Koh et al. (2011)