› 2hg2

X-ray diffraction
2.2Å resolution

Structure of Lactaldehyde Dehydrogenase

Released:
DOI: 10.2210/pdb2hg2/pdb
PDB entry 2hg2 coloured by chain, front view.
PDB entry 2hg2 coloured by chain, side view.
PDB entry 2hg2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity.
Di Costanzo L, Gomez GA, Christianson DW
J Mol Biol 366 481-93 (2007)
PMID: 17173928

Function and Biology Details

Reactions catalysed: 
Glycolaldehyde + NAD(+) + H(2)O = glycolate + NADH
(S)-lactaldehyde + NAD(+) + H(2)O = (S)-lactate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactaldehyde dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 479 amino acids
Theoretical weight: 52.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P25553 (Residues: 1-479; Coverage: 100%)
Gene names: JW1412, ald, aldA, b1415
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P6422
Unit cell:
a: 143.94Å b: 143.94Å c: 108.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biotechnological routes based on lactic acid production from biomass.
Gao et al. (2011)
2 more

5 mentions without citation

Crystal structure of lactaldehyde dehydrogenase from Escherichia coli and inferences regarding substrate and cofactor specificity.
Di Costanzo et al. (2007)
4 more