› 2hdp

Solution NMR

Solution Structure of Hdm2 RING Finger Domain

Released:
DOI: 10.2210/pdb2hdp/pdb
PDB entry 2hdp coloured by chain, ensemble of 20 models, front view.
PDB entry 2hdp coloured by chain, ensemble of 20 models, side view.
PDB entry 2hdp coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the Hdm2 C2H2C4 RING, a domain critical for ubiquitination of p53.
Kostic M, Matt T, Martinez-Yamout MA, Dyson HJ, Wright PE
J Mol Biol 363 433-50 (2006)
PMID: 16965791

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Mdm2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 63 amino acids
Theoretical weight: 7.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00987 (Residues: 429-491; Coverage: 13%)
Gene name: MDM2
Sequence domains: Zinc finger, C3HC4 type (RING finger)
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

22 review citations

The first 30 years of p53: growing ever more complex.
Levine et al. (2009)
21 more

5 mentions without citation

Induction of tumor apoptosis through a circular RNA enhancing Foxo3 activity.
Du et al. (2017)
4 more