› 2hdd

X-ray diffraction
1.9Å resolution

ENGRAILED HOMEODOMAIN Q50K VARIANT DNA COMPLEX

Released:
DOI: 10.2210/pdb2hdd/pdb
PDB entry 2hdd coloured by chain, front view.
PDB entry 2hdd coloured by chain, side view.
PDB entry 2hdd coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Engrailed (Gln50-->Lys) homeodomain-DNA complex at 1.9 A resolution: structural basis for enhanced affinity and altered specificity.
Tucker-Kellogg L, Rould MA, Chambers KA, Ades SE, Sauer RT, Pabo CO
Structure 5 1047-54 (1997)
PMID: 9309220

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115338 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Segmentation polarity homeobox protein engrailed Chains: A, B
Molecule details ›
Chains: A, B
Length: 61 amino acids
Theoretical weight: 7.43 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P02836 (Residues: 454-512; Coverage: 11%)
Gene names: CG9015, en
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*TP*TP*TP*TP*GP*CP*CP*AP*TP*GP*TP*AP*AP*TP*CP*CP*C P*CP*GP*GP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.39 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*AP*TP*CP*CP*GP*GP*GP*GP*AP*TP*TP*AP*CP*AP*TP*GP*G P*CP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.5 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 127.72Å b: 45.34Å c: 72.47Å
α: 90° β: 119.53° γ: 90°
R-values:
R R work R free
0.205 0.205 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
4 more

24 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
23 more