PDBe 2h9v

X-ray diffraction
3.1Å resolution

Structural basis for induced-fit binding of Rho-kinase to the inhibitor Y27632

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho-associated protein kinase 2 Chain: A
Molecule details ›
Chain: A
Length: 402 amino acids
Theoretical weight: 45.78 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q28021 (Residues: 18-417; Coverage: 29%)
Gene name: ROCK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P6222
Unit cell:
a: 90.781Å b: 90.781Å c: 341.279Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.232 0.277
Expression system: Spodoptera frugiperda