PDBe 2h7h

X-ray diffraction
2.3Å resolution

Crystal structure of the JUN BZIP homodimer complexed with AP-1 DNA

Released:
Entry authors: Rajan SS, Borovilos M, Cuff ME, Kim Y

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Viral jun-transforming protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 62 amino acids
Theoretical weight: 7.32 KDa
Source organism: Avian sarcoma virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05411 (Residues: 210-271; Coverage: 22%)
Gene name: JUN
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
5'-D(*CP*GP*TP*CP*GP*AP*TP*GP*AP*CP*TP*CP*AP*TP*CP*GP*AP*CP*G)-3' Chain: X
Molecule details ›
Chain: X
Length: 19 nucleotides
Theoretical weight: 5.81 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*CP*GP*TP*CP*GP*AP*TP*GP*AP*GP*TP*CP*AP*TP*CP*GP*AP*CP*G)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 19 nucleotides
Theoretical weight: 5.85 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 42.258Å b: 74.203Å c: 58.303Å
α: 90° β: 111.25° γ: 90°
R-values:
R R work R free
0.224 0.218 0.274
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided