PDBe 2h6d

X-ray diffraction
1.85Å resolution

Protein Kinase Domain of the Human 5'-AMP-activated protein kinase catalytic subunit alpha-2 (AMPK alpha-2 chain)

Released:

Function and Biology Details

Reactions catalysed:
ATP + [acetyl-CoA carboxylase] = ADP + [acetyl-CoA carboxylase] phosphate. 
ATP + a protein = ADP + a phosphoprotein. 
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-AMP-activated protein kinase catalytic subunit alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P54646 (Residues: 6-279; Coverage: 50%)
Gene names: AMPK, AMPK2, PRKAA2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 39.454Å b: 67.41Å c: 50.493Å
α: 90° β: 91.3° γ: 90°
R-values:
R R work R free
0.19 0.188 0.227
Expression system: Escherichia coli BL21