› 2h5s

X-ray diffraction
1.28Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase SHV-1 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.91 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: P0AD64 (Residues: 22-286; Coverage: 100%)
Gene names: bla, blaSHV-1, shv1
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand SA2 1 x SA2
Ligand MA4 2 x MA4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 49.81Å b: 55.22Å c: 84.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.154 0.167
Expression system: Escherichia coli str. K-12 substr. DH10B

Quick links

Citations

5 review citations

Current challenges in antimicrobial chemotherapy: focus on ß-lactamase inhibition.
Bebrone et al. (2010)
4 more

8 mentions without citation

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
7 more