PDBe 2h5k

X-ray diffraction
3.25Å resolution

Crystal Structure of Complex Between the Domain-Swapped Dimeric Grb2 SH2 Domain and Shc-Derived Ligand, Ac-NH-pTyr-Val-Asn-NH2

Primary publication:
Structural and energetic aspects of Grb2-SH2 domain-swapping.
Arch. Biochem. Biophys. 462 47-53 (2007)
PMID: 17466257

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Shc-Derived Ligand Chain: C
Molecule details ›
Chain: C
Length: 5 amino acids
Theoretical weight: 498 Da
Source organism: Synthetic construct
Expression system: Not provided
Growth factor receptor-bound protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 13.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P62993 (Residues: 53-162; Coverage: 51%)
Gene names: ASH, GRB2
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6222
Unit cell:
a: 94.859Å b: 94.859Å c: 139.236Å
α: 90° β: 90° γ: 120°
R R work R free
0.247 0.247 0.3
Expression systems:
  • Not provided
  • Escherichia coli