PDBe 2h34

X-ray diffraction
2.8Å resolution

Apoenzyme crystal structure of the tuberculosis serine/threonine kinase, PknE

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknE Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.06 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WI77 (Residues: 2-16, 18-289; Coverage: 51%)
Gene names: MTCY28.05, Rv1743, pknE
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P65
Unit cell:
a: 77.078Å b: 77.078Å c: 221.024Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.216 0.265
Expression system: Escherichia coli BL21(DE3)