PDBe 2gwd

X-ray diffraction
2.09Å resolution

Crystal structure of plant glutamate cysteine ligase in complex with Mg2+ and L-glutamate

Released:
Source organism: Brassica juncea

Function and Biology Details

Reaction catalysed:
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--cysteine ligase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 50.97 KDa
Source organism: Brassica juncea
Expression system: Escherichia coli
UniProt:
  • Canonical: O23736 (Residues: 66-514; Coverage: 87%)
Gene names: ECS1, GSH1
Sequence domains: Glutamate-cysteine ligase family 2(GCS2)
Structure domains: Creatine Kinase; Chain A, domain 2

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6122
Unit cell:
a: 54.81Å b: 54.81Å c: 518.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.175 0.227
Expression system: Escherichia coli