› 2gmr

X-ray diffraction
2.5Å resolution

Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn

Released:
DOI: 10.2210/pdb2gmr/pdb
PDB entry 2gmr coloured by chain, front view.
PDB entry 2gmr coloured by chain, side view.
PDB entry 2gmr coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Proton uptake in the reaction center mutant L210DN from Rhodobacter sphaeroides via protonated water molecules.
Hermes S, Stachnik JM, Onidas D, Remy A, Hofmann E, Gerwert K
Biochemistry 45 13741-9 (2006)
PMID: 17105193

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142934 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 281 amino acids
Theoretical weight: 31.35 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y8 (Residues: 2-282; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 307 amino acids
Theoretical weight: 34.4 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y9 (Residues: 2-308; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 28.07 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: P0C0Y7 (Residues: 1-260; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand U10 2 x U10
Ligand LDA 4 x LDA
Ligand FE2 1 x FE2
Ligand SPN 1 x SPN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 76.85Å b: 134.72Å c: 141.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.25
Expression system: Cereibacter sphaeroides

Quick links

Citations

3 review citations

Time-resolved methods in biophysics. 10. Time-resolved FT-IR difference spectroscopy and the application to membrane proteins.
Radu et al. (2009)
2 more