› 2gml

X-ray diffraction
2.6Å resolution

Crystal Structure of Catalytic Domain of E.coli RluF

Released:
DOI: 10.2210/pdb2gml/pdb
PDB entry 2gml coloured by chain, front view.
PDB entry 2gml coloured by chain, side view.
PDB entry 2gml coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Domain organization and crystal structure of the catalytic domain of E.coli RluF, a pseudouridine synthase that acts on 23S rRNA.
Sunita S, Zhenxing H, Swaathi J, Cygler M, Matte A, Sivaraman J
J Mol Biol 359 998-1009 (2006)
PMID: 16712869

Function and Biology Details

Reaction catalysed: 
23S rRNA uridine(2604) = 23S rRNA pseudouridine(2604)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual-specificity RNA pseudouridine synthase RluF Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 26.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32684 (Residues: 66-290; Coverage: 78%)
Gene names: JW3982, b4022, rluF, yjbC
Sequence domains: RNA pseudouridylate synthase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 65.714Å b: 65.714Å c: 215.914Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure, dynamics, and function of RNA modification enzymes.
Ishitani et al. (2008)
1 more

2 mentions without citation

Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome.
Alian et al. (2009)
1 more