PDBe 2gih

X-ray diffraction
2.5Å resolution

Q138F HincII bound to cognate DNA GTCGAC and Ca2+

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type-2 restriction enzyme HincII Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.84 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 2-258; Coverage: 100%)
Gene name: hincIIR
Sequence domains: Restriction endonuclease HincII
Structure domains: ECO RV Endonuclease; Chain A
5'-D(*GP*CP*CP*GP*GP*TP*CP*GP*AP*CP*CP*GP*GP*C)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: I41
Unit cell:
a: 127.861Å b: 127.861Å c: 81.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.283
Expression systems:
  • Escherichia coli
  • Not provided