PDBe 2gie

X-ray diffraction
2.6Å resolution

HincII bound to cognate DNA GTTAAC

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type-2 restriction enzyme HincII Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 257 amino acids
Theoretical weight: 29.82 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17743 (Residues: 2-258; Coverage: 100%)
Gene name: hincIIR
Sequence domains: Restriction endonuclease HincII
Structure domains: ECO RV Endonuclease; Chain A
5'-D(*GP*CP*CP*GP*GP*TP*TP*AP*AP*CP*CP*GP*G)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 13 nucleotides
Theoretical weight: 3.99 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: I212121
Unit cell:
a: 67.243Å b: 175.472Å c: 253.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.238 0.306
Expression systems:
  • Escherichia coli
  • Not provided