Function and Biology

Structural comparison of the oxidized ternary electron transfer complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans with the substrate-reduced, copper free complex at 1.9 A resolution.

Source organism: Paracoccus denitrificans
Biochemical function: oxidoreductase activity
Biological process: oxidation-reduction process
Cellular component: periplasmic space

EC 1.4.9.1: Methylamine dehydrogenase (amicyanin)

Reaction catalysed:
Methylamine + H(2)O + 2 oxidized [amicyanin] = formaldehyde + NH(3) + 2 reduced [amicyanin].
Comments:
  • The enzyme oxidizes aliphatic monoamines and diamines, histamine and ethanolamine, but not secondary and tertiary amines, quaternary ammonium salts or aromatic amines.
  • Formerly EC 1.4.98.1 and EC 1.4.99.3.
Systematic name:
Methylamine:amicyanin oxidoreductase (deaminating)
Alternative Name(s):
  • Amine:(acceptor) oxidoreductase (deaminating)
  • Methylamine dehydrogenase
  • Primary-amine dehydrogenase
  • MADH
  • Primary-amine:(acceptor) oxidoreductase (deaminating)
  • Amine dehydrogenase

Sequence families

CATH Protein families (Pfam)
PF13442
Domain description: Cytochrome C oxidase, cbb3-type, subunit III
Occurring in:
  1. Cytochrome c-L
1 copy of Pfam domain PF13442 (Cytochrome C oxidase, cbb3-type, subunit III) in Cytochrome c-L in PDB 2gc4.

PF00127
Domain description: Copper binding proteins, plastocyanin/azurin family
Occurring in:
  1. Amicyanin
1 copy of Pfam domain PF00127 (Copper binding proteins, plastocyanin/azurin family) in Amicyanin in PDB 2gc4.

PF06433
Domain description: Methylamine dehydrogenase heavy chain (MADH)
Occurring in:
  1. Methylamine dehydrogenase heavy chain
1 copy of Pfam domain PF06433 (Methylamine dehydrogenase heavy chain (MADH)) in Methylamine dehydrogenase heavy chain in PDB 2gc4.

PF02975
Domain description: Methylamine dehydrogenase, L chain
Occurring in:
  1. Methylamine dehydrogenase light chain
1 copy of Pfam domain PF02975 (Methylamine dehydrogenase, L chain) in Methylamine dehydrogenase light chain in PDB 2gc4.

CATH InterPro annotations
IPR002386
Domain description: Amicyanin/Pseudoazurin
Occurring in:
  1. Amicyanin
IPR009056
Domain description: Cytochrome c-like domain
Occurring in:
  1. Cytochrome c-L
IPR013504
Domain description: Methylamine/Aralkylamine dehydrogenase light chain, C-terminal domain
Occurring in:
  1. Methylamine dehydrogenase light chain
IPR001235
Domain description: Blue (type 1) copper protein, plastocyanin-type
Occurring in:
  1. Amicyanin
IPR008972
Domain description: Cupredoxin
Occurring in:
  1. Amicyanin
IPR009153
Domain description: Cytochrome cL
Occurring in:
  1. Cytochrome c-L
IPR015943
Domain description: WD40/YVTN repeat-like-containing domain
Occurring in:
  1. Methylamine dehydrogenase heavy chain
IPR013476
Domain description: Methylamine dehydrogenase heavy chain
Occurring in:
  1. Methylamine dehydrogenase heavy chain
IPR000923
Domain description: Blue (type 1) copper domain
Occurring in:
  1. Amicyanin
IPR028871
Domain description: Blue (type 1) copper protein, binding site
Occurring in:
  1. Amicyanin
IPR013475
Domain description: Amicyanin, Paracoccus/Methylobacterium
Occurring in:
  1. Amicyanin
IPR011044
Domain description: Quinoprotein amine dehydrogenase, beta chain-like
Occurring in:
  1. Methylamine dehydrogenase heavy chain
IPR035668
Domain description: Amicyanin
Occurring in:
  1. Amicyanin
IPR004229
Domain description: Methylamine dehydrogenase light chain
Occurring in:
  1. Methylamine dehydrogenase light chain
IPR009451
Domain description: Amine dehydrogenase heavy chain
Occurring in:
  1. Methylamine dehydrogenase heavy chain

Structure domains

CATH CATH domains
2.60.40.420
Class: Mainly Beta
Architecture: Sandwich
Topology: Immunoglobulin-like
Homology: Cupredoxins - blue copper proteins
Occurring in:
  1. Amicyanin
1 copy of CATH domain 2.60.40.420 (Immunoglobulin-like) in Amicyanin in PDB 2gc4.
1.10.760.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Cytochrome Bc1 Complex; Chain D, domain 2
Homology: Cytochrome c
Occurring in:
  1. Cytochrome c-L
1 copy of CATH domain 1.10.760.10 (Cytochrome Bc1 Complex; Chain D, domain 2) in Cytochrome c-L in PDB 2gc4.
2.60.30.10
Class: Mainly Beta
Architecture: Sandwich
Topology: Electron Transport Ethylamine Dehydrogenase
Homology: Electron Transport Ethylamine Dehydrogenase
Occurring in:
  1. Methylamine dehydrogenase light chain
1 copy of CATH domain 2.60.30.10 (Electron Transport Ethylamine Dehydrogenase) in Methylamine dehydrogenase light chain in PDB 2gc4.
2.130.10.10
Class: Mainly Beta
Architecture: 7 Propellor
Topology: Methylamine Dehydrogenase; Chain H
Homology: YVTN repeat-like/Quinoprotein amine dehydrogenase
Occurring in:
  1. Methylamine dehydrogenase heavy chain
1 copy of CATH domain 2.130.10.10 (Methylamine Dehydrogenase; Chain H) in Methylamine dehydrogenase heavy chain in PDB 2gc4.
SCOP SCOP annotations
46627
Class: All alpha proteins
Fold: Cytochrome c
Superfamily: Cytochrome c
Occurring in:
  1. Cytochrome c-L
1 copy of SCOP domain 46627 (monodomain cytochrome c) in Cytochrome c-L in PDB 2gc4.
49504
Class: All beta proteins
Fold: Cupredoxin-like
Superfamily: Cupredoxins
Occurring in:
  1. Amicyanin
1 copy of SCOP domain 49504 (Plastocyanin/azurin-like) in Amicyanin in PDB 2gc4.
57562
Class: Small proteins
Fold: Methylamine dehydrogenase, L chain
Superfamily: Methylamine dehydrogenase, L chain
Occurring in:
  1. Methylamine dehydrogenase light chain
1 copy of SCOP domain 57562 (Methylamine dehydrogenase, L chain) in Methylamine dehydrogenase light chain in PDB 2gc4.
50970
Class: All beta proteins
Fold: 7-bladed beta-propeller
Superfamily: YVTN repeat-like/Quinoprotein amine dehydrogenase
Occurring in:
  1. Methylamine dehydrogenase heavy chain
1 copy of SCOP domain 50970 (Methylamine dehydrogenase, H-chain) in Methylamine dehydrogenase heavy chain in PDB 2gc4.