PDBe 2gc4

X-ray diffraction
1.9Å resolution

Structural comparison of the oxidized ternary electron transfer complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans with the substrate-reduced, copper free complex at 1.9 A resolution.

Released:
Source organism: Paracoccus denitrificans
Entry authors: Chen Z, Durley R, Davidson VL, Mathews FS

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Amicyanin Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 105 amino acids
Theoretical weight: 11.51 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Cytochrome c-L Chains: D, H, L, P
Molecule details ›
Chains: D, H, L, P
Length: 147 amino acids
Theoretical weight: 16.27 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P29899 (Residues: 23-169; Coverage: 95%)
Gene name: moxG
Sequence domains: Cytochrome C oxidase, cbb3-type, subunit III
Structure domains: Cytochrome c-like domain
Methylamine dehydrogenase heavy chain Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 386 amino acids
Theoretical weight: 42.45 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P29894 (Residues: 32-417; Coverage: 99%)
Gene name: mauB
Sequence domains: Methylamine dehydrogenase heavy chain (MADH)
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Methylamine dehydrogenase light chain Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 131 amino acids
Theoretical weight: 14.21 KDa
Source organism: Paracoccus denitrificans
UniProt:
  • Canonical: P22619 (Residues: 58-188; Coverage: 100%)
Gene name: mauA
Sequence domains: Methylamine dehydrogenase, L chain
Structure domains: Methylamine/Aralkylamine dehydrogenase light chain

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 81.368Å b: 188.979Å c: 128.836Å
α: 90° β: 99.74° γ: 90°
R-values:
R R work R free
0.173 0.173 0.197