› 2gbm

X-ray diffraction
1.55Å resolution

Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin

Released:
DOI: 10.2210/pdb2gbm/pdb
PDB entry 2gbm coloured by chain, front view.
PDB entry 2gbm coloured by chain, side view.
PDB entry 2gbm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of ubiquitin insertion mutants support site-specific reflex response to insertions hypothesis.
Ferraro DM, Ferraro DJ, Ramaswamy S, Robertson AD
J Mol Biol 359 390-402 (2006)
PMID: 16647719

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 84 amino acids
Theoretical weight: 9.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-642, 643-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand ARS 3 x ARS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 48.817Å b: 49.786Å c: 123.147Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Thermodynamic analysis of an antagonistic folding-unfolding equilibrium between two protein domains.
Cutler et al. (2007)
3 more

1 mention without citation

The structure of the N-terminus of kindlin-1: a domain important for alphaiibbeta3 integrin activation.
Goult et al. (2009)